Generator

Part:BBa_K1896010

Designed by: Bob Van Hove, Maarten Van Brempt   Group: iGEM16_UGent_Belgium   (2016-10-13)


mGFPuv2 generator

This part constitutively expresses mGFPuv2, a monomeric GFP variant optimised for excitation by UV light (360-400nm). The J23119 promoter is a strong consensus promoter and expression is high enough for colonies to display visible fluorescence in daylight.

  • Colonies of E. coli containing BBa_K1896010.

  • The same colonies imaged under long-wave UV light.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None